Nischay Mishra, PhD

Associate Research Scientist
Phone: 212.342.5176
Fax: 212.342.9044
Email: nm2641@columbia.edu






EDUCATION & TRAINING

Lucknow University, Lucknow, India, BSc (Biology), 2001
Kanpur University, Kanpur, India, MSc (Biotechnology), 2004
National Institute of Virology (University of Pune), India, PhD, Biotechnology, 2011

RESEARCH INTERESTS

Rapid and broad-spectrum molecular and serological techniques for identifying unknown infectious agents
Genomic and evolutionary phylogenetic analysis of emerging DNA and RNA viruses
Genome mutation and changes in virulence and transmissibility of viral agents
Association of disease and disease progression with changes in the microbiome

BIO

Dr. Mishra is a virologist and molecular biologist with over 10 years of experience in high throughput sequencing, viral metagenomics, serology, viral epidemiology, diagnostics and bioinformatics. His achievements include the identification of a novel polyomavirus associated with disease in a transplant recipient exposed to Superstorm Sandy floodwaters, assistance in the discovery of Middle East Respiratory virus in bats and camels in the Kingdom of Saudi Arabia, and contribution to the development of the VirCapSeq-VERT, a diagnostic platform that screens for all known vertebrate viruses present in a sample and also detects novel viruses with up to 60% genome divergence from known sequence. Dr. Mishra was also a member of team which recently developed first diagnostic platform “BacCapSeq” that can simultaneously screen for all known human pathogenic bacteria as well as markers for virulence and antibiotic resistance.

Mishra is currently focusing on development of molecular and serological platforms to specific diagnose Zika virus, dengue virus, Chikungunya virus and other arboviruses and he led the team and recently obtained a FDA Emergency User Authorization (EUA) application for an economic and sensitive method: CII-ArboViroPlex assay to detect Zika and other arboviruses. He has also developed a multiplex serological platform; Serochips-peptide arrays for the identification of discriminatory set of immunoreactive epitopes, and same technology was also used to develop the first multiplex diagnostic serologic platforms for arboviral diseases, acute encephalitis and tick-borne diseases.

PUBLICATIONS ​

Allicock OM,* Guo C, Uhlemann AC, Whittier S, Chauhan LV, Garcia J, Price A, Morse S, Mishra N, Briese T, Lipkin, W. I. (2018). BacCapSeq: A platform for diagnosis and characterization of bacterial 1 infections. doi: mBio 10.1128/mBio.02007-18

Arya R., Mishra N., Biswas K., Arankalle V.A. (2018). Association of Toll‐like receptor 4 Polymorphism with Hepatitis E virus infected Indian patients. J Virol hep. doi:10.1111/jvh.12980

Mishra, N., Caciula, A., Price, A., Thakkar, R., Ng, J., Chauhan, L. V.,...Lipkin, W. I. (2018). Diagnosis of Zika Virus Infection by Peptide Array and Enzyme-Linked Immunosorbent Assay. MBio, 9(2). doi:10.1128/mBio.00095-18

Tokarz, R.*, Mishra, N.*, Tagliafierro, T., Sameroff, S., Caciula, A., Chauhan, L.,...Lipkin, W. I. (2018). A multiplex serologic platform for diagnosis of tick-borne diseases. Sci Rep, 8(1), 3158. doi:10.1038/s41598-018-21349-2

Del-Pozo, J.*, Mishra, N.*, Kabuusu, R., Cheetham, S., Eldar, A., Bacharach, E.,...Ferguson, H. W. (2017). Syncytial Hepatitis of Tilapia ( Oreochromis niloticus L.) is Associated With Orthomyxovirus-Like Virions in Hepatocytes. Vet Pathol, 54(1), 164-170. doi:10.1177/0300985816658100

Kembou Tsofack, J. E., Zamostiano, R., Watted, S., Berkowitz, A., Rosenbluth, E., Mishra, N.,...Bacharach, E. (2017). Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription-PCR. J Clin Microbiol, 55(3), 759-767. doi:10.1128/JCM.01808-16

Nagy-Szakal, D., Williams, B. L., Mishra, N., Che, X., Lee, B., Bateman, L.,...Lipkin, W. I. (2017). Fecal metagenomic profiles in subgroups of patients with myalgic encephalomyelitis/chronic fatigue syndrome. Microbiome, 5(1), 44. doi:10.1186/s40168-017-0261-y

Nicholson, P., Fathi, M. A., Fischer, A., Mohan, C., Schieck, E., Mishra, N.,...Jores, J. (2017). Detection of Tilapia Lake Virus in Egyptian fish farms experiencing high mortalities in 2015. J Fish Dis, 40(12), 1925-1928. doi:10.1111/jfd.12650

Bacharach, E.*, Mishra, N.*, Briese, T., Zody, M. C., Kembou Tsofack, J. E., Zamostiano, R.,...Lipkin, W. I. (2016). Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia. MBio, 7(2), e00431-00416. doi:10.1128/mBio.00431-16

Briese T, Kapoor A, Mishra N, Jain K, Kumar A, Jabado OJ, Lipkin WI. Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis. MBio. 2015 Sept-Oct; 6(5). doi:10.1128/mBio.01491-15

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